Antibiotic Discs

Oxidase Disc

Recommended for detection of oxidase production by microorganisms like Neisseria, Alcaligenes, Aeromonas, Vibrio, Campylobacter and Pseudomonas which give positive reactions and for excluding Enterobacteriaceae, which give negative reactions.

Range	Antibiotic Discs
Composition	Sterile filter paper discs impregnated with N,N-dimethyl-p-phenylenediamine oxalate, ascorbic acid and α-naphthol.
Appearance	Filter paper discs of 10 mm diameter.
Procedure	Touch and spread a well isolated colony on the oxidase disc. The reaction is observed within 5-10 seconds at 25-30°C; a change later than 10 seconds or no change at all is considered a negative reaction.
Storage	BioScien Oxidase Disc should be stored between 2 to 8 °C in a firmly closed container. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Quality control	Typical oxidase reaction given by 18-48 hour culture observed within 5-10 seconds at 25-30°C. Pseudomonas aeruginosa ATCC 27853: positive, deep purplish blue colouration of disc; Neisseria gonorrhoeae ATCC 19424: positive, deep purplish blue colouration of disc; Escherichia coli ATCC 25922: negative, purplish blue colouration after 10 sec / no colour change; Staphylococcus aureus subsp. aureus ATCC 25923: negative, no colour change.
Pack sizes	1 option