Coagulation Reagents

Fibrinogen-Lyo

Diagnostic reagent for the in-vitro quantitative determination of plasma Fibrinogen using Clauss Clotting Time method in both automatic and manual systems.

Range	Coagulation Reagents
Method	Clauss clotting time
Type of specimen	Plasma. The blood should be collected by careful venipuncture and transferred into a tube containing trisodium citrate (0.11 mol/L, 3.2%); mix blood with the anticoagulant at a ratio of 9:1, avoiding foam formation during mixing. For occasional patients with a haematocrit below 20% or above 55%, this ratio must be readjusted to ensure a valid result.
Specimen handling	Centrifuge immediately for 15 minutes at 1500–3000 rpm (approximately 1500 g) and transfer the plasma into a clean test tube; it should be ensured that the plasma is free from platelets. Cap the test tubes to prevent deterioration of samples. Plasma must be tested immediately; if the specimens are held at 2–4 °C they may be tested within 3 hours. Oxalated plasma may induce prolonged clotting times. Incorrect mixture of blood and trisodium citrate is a potential source of errors. Icteric, lipemic and haemolytic samples can modify the results in an unforeseeable way.
Storage	Stable until the expiry date on the label when properly stored refrigerated at 2–8 °C (do not freeze). Bovine thrombin: reconstitute individual vials with the volume of distilled water stated on the vial label and mix; once reconstituted, stable up to 15 days at 2–8 °C and for 4 hours at room temperature. Buffer: open vial stable up to 30 days at 2–8 °C. Calibrator: reconstitute individual vials with the volume of distilled water stated on the vial label and mix for 2 minutes; reconstituted calibrator is stable for 2 hours at room temperature and 6 hours at 2–8 °C.
Catalogue numbers	1