Bacteriology Media
Violet Red Bile Lactose Agar
For the detection and colony counting of coliform bacteria from food and animal feed, water and other materials.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula in g/L: Enzymatic Digest of Animal Tissues 7; Yeast Extract 3; Bile salts mixture 1.5; Sodium chloride 5; Lactose 10; Neutral red 0.03; Crystal violet 0.002; Agar 15; pH 7.2 ± 0.2 at 25°C |
| Preparation | Suspend 50.0 grams in 1000 ml purified / distilled water. Adjust pH to pH 7.2 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. Afterwards do not boil for more than 2 minutes. Mix well and pour into sterile Petri plates. |
| Storage | BioScien Violet Red Bile Lactose Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | BioScien Violet Red Bile Lactose Agar is Light beige to reddish-beige homogeneous free flowing powder. Prepared medium is slightly opalescent, reddish-purple gel forms in Petri plates. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | For clinical samples follow appropriate techniques for handling specimens as per established guidelines (11,12). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (9,10,13). After use, contaminated materials must be sterilized by autoclaving before discarding. Type of specimen: Clinical samples - stool; Food and Dairy samples |
| Pack sizes | 3 options |
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