Arena BioScien
Bacteriology Media
Charcoal Agar Base
BS.1/NZ01.100.0100
Fits your perfection
Bacteriology Media
Charcoal Agar Base
Used for cultivation of Bordetella pertussis, for vaccine production and also for stock culture maintenance.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium g/l: Peptone 10.0; Yeast extract 3.50; Starch, soluble 10.0; Charcoal 4.0; Sodium chloride 5.0; HM infusion B from 500g# 12.0; Agar 18.0. Final pH 7.0 ± 0.2. ** Formula adjusted, standardized to suit performance parameters. # Equivalent to beef heart, infusion from |
| Preparation | Suspend 31.25 grams in 450 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50° and aseptically add sterile 10% of defibrinated blood and rehydrated contents of one vial of Bos Selective Supplement (FD004). Mix well and pour into sterile Petri plates. For Haemophilus species, the medium can be converted to Chocolate Agar. |
| Storage | BioScien Charcoal Agar Base Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Charcoal Agar Base is Grey to greyish black homogeneous free flowing powder. Colour and Clarity of prepared medium Black coloured, opaque gel with undissolved black particles forms in Petri plates. |
| Pack sizes | 3 options |
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