Arena BioScien
Bacteriology Media
Columbia Blood Agar Base
BS.1/CMP01.100.0100
Fits your perfection
Bacteriology Media
Columbia Blood Agar Base
For preparation of blood agar, chocolate agar and for preparation of various selective and identification media and isolation of organisms from clinical and non clinical samples.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptone 23.00 gm; Corn starch 1.00 gm; Sodium chloride 5.000 gm; Agar 15.00 gm. Final pH 7.3+ 0.2 at 25 °C |
| Preparation | Suspend 44.0 grams of in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C before adding heat sensitive compounds. For Blood Agar: Add 5% v/v sterile defibrinated sheep blood to sterile cool base. For Chocolate Agar: Add 10% v/v sterile defibrinated sheep blood to sterile cool base. Heat to 80°C for 10 minutes with constant agitation. The medium can be made selective by adding different antimicrobials to sterile base. For Brucella species: Add rehydrated contents of 1 vial of NPBCVN Selective Supplement (FD005) to 500 ml sterile molten base. For Campylobacter species: Add rehydrated contents of 1 vial of Blaser-Wang Selective Supplement (FD006) or Butzler Selective Supplement (FD007) or Skirrow Selective Supplement (FD008) or VTCA Selective Supplement (FD090) or Butzler VI Selective Supplement (FD106) to 500 ml sterile molten base along with rehydrated contents of 1 vial of Minerals Growth Supplement (FD009) and 5-7% v/v horse or sheep blood. For Gardnerella species: Add rehydrated contents of 1 vial of GNA Selective Supplement (FD056) to 500 ml sterile molten base. For Cocci: Add rehydrated contents of 1 vial of NC Selective Supplement (FD030) or NNP Selective Supplement (FD031) or CO Selective Supplement (FD119) to 500 ml sterile molten base. |
| Storage | BioScien Columbia Blood Agar Base should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Columbia Blood Agar Base is Cream to yellow homogeneous free flowing powder. prepared medium Basal medium: Light amber coloured clear to slightly opalescent gel. After addition of 5%w/v sterile defibrinated blood: Cherry red coloured opaque gel forms in Petri plates. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Pack sizes | 3 options |
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