Arena BioScien
Bacteriology Media
Coagulase Reagent
BS.1/CG01.005.0005
Fits your perfection
Bacteriology Media
Coagulase Reagent
Lyophilized rabbit plasma is used for the qualitative detection of the coagulase enzyme produced by Staphylococcus aureus
| Range | Bacteriology Media |
|---|---|
| Composition | REAGENT COMPOSITION: Coagulase Plasma is lyophilized rabbit plasma to which EDTA is added as an anti-coagulant. EDTA is not consumed by bacteria, thus will not cause false positive coagulase reactions by bacteria that can utilize citrate. |
| Preparation | Rehydrate lyophilized Coagulase Plasma with 0.5ml sterile distilled water |
| Procedure | A. Tube Coagulase test: 1. Using a sterile 1 ml pipette, add 0.5 ml of the reconstituted plasma to a test tube. 2. Using a sterile loop emulsify 2-4 colonies of the test organism into the plasma. 3. Mix gently and DON’T SHAKE. 4. Incubate for 4 hours in a water bath at 37°C. 5. After 1 hour check for clot formation by gently tipping the tube onto its side. If no clot formation is evident examine every 30 minutes up to the 4 hour limit. 6. If there is no clot after 4 hours incubation, reincubate the test at room temperature for the remaining time period and examine for clot formation at 24 hours. Do not reincubate tests that have already produced a clot at 4 hours as some strains of S. aureus will produce a fibrinolysin that may lyse the clots after further incubation. 7. Record the results. B. Slide Coagulase Test: (For detection of Bound coagulase or Clumping Factor) 1. Place a drop of the reconstituted plasma and a drop of saline side by side on a clean, dry glass slide. 2. Emulsify a loopful of the colonies to be tested into the drop of plasma and into the saline. 3. Observe for visible clumps for up to one minute. 4. Record results. |
| Storage | BioScien Coagulase Reagent is stable until expiration date stated on label when properly stored at 2-8°C for non-reconstituted and unopened vials. Aliquots may be tightly capped and frozen at -20°C or below for up to 1 month or refrigerate at 2-8°C for 5 days. Frozen aliquots of Coagulase Plasma should not be refrozen after thawing. Deterioration: The color of BioScien Coagulase Reagent should not be used if the plasma is clotted upon rehydration, the product is contaminated, the expiration has passed, or there is additional sign of deterioration. |
| Specimen | The test isolate should be pure and has the following characteristics of Staphylococcus aureus: Appropriate morphology on the isolation medium.; Gram positive-cocci; Catalase positive. |
| Pack sizes | 4 options |
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