Bacteriology Media
Violet Red Bile Agar
Violet Red Bile Agar is selective medium used for the isolation, detection and enumeration of coli-aerogenes bacteria in water, milk, and other dairy food products and also from clinical samples.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptic digest of animal tissue 7.000 gm.; Yeast extract 3.000 gm.; Sodium chloride 5.000 gm.; Bile salts mixture 1.500 gm.; Lactose 10.00 gm.; Neutral red 0.030 gm.; Crystal violet 0.002 gm.; Agar 15.00 gm.; Final pH 7.4 ± 0.2 at 25°C |
| Preparation | Suspend 41.53 grams in 1000 ml distilled water. Adjust pH to 7.4 ± 0.2 at 25°C. Heat with stirring to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45°C and pour into sterile Petri plates containing the inoculum. If desired, the medium can be sterilized by autoclaving at 15 lbs pressure at 15lbs pressure (121°C) for 15 minutes. |
| Storage | BioScien Violet Red Bile agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Violet Red Bile agar is Light yellow to pink homogeneous free flowing powder. Prepared Medium is Reddish purple coloured clear to slightly opalescent gel forms in Petri plates. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical samples; Food; Water |
| Pack sizes | 3 options |
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