Bacteriology Media
C.L.E.D. Agar w/Bromo Thymol Blue
Medical laboratories media for the isolation, enumeration, and identification of urinary pathogens on the basis of lactose fermentation, while controlling the swarming of Proteus spp.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula in g/L: Lactose 10.0; Peptone 4.00; Trypton 4.00; Beef Extract 3.00; L-Cystine 0.128; Bromothymol Blue 0.02; Agar 15.00. Final pH 7.3 ± 0.2 at 25°C |
| Preparation | Hydrated CLED Agar media is prepared by suspend 36.15 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121°C for 15 minutes. Cool to 50°C, mix well and dispense into plates. When the medium is solidified, invert the plates to avoid excess moisture. |
| Storage | BioScien CLED Agar dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 8-15°C. |
| Appearance | The color of BioScien CLED Agar medium is Blue/Green, dehydrated medium is Cream to yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium is Blue/green colored gel, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Urine. Urine samples to be collected in sterile container, specimens is stable in urine for 2 days at 15-25°C and 6 days at 2-8°C (freeze for longer storage). Note: for patient preparation follow Medical laboratory instruction |
| Pack sizes | 3 options |
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