Bacteriology Media
Campylobacter Agar Base
Designed for selective isolation of Campylobacter species from faecal specimens, food and environmental specimens.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Proteose peptone 15.00 gm; Liver digest 2.500 gm; Yeast extract 5.000 gm; Sodium chloride 5.000 gm; Agar 12.00 gm; Final pH 7.4 + 0.2 at 25 °C |
| Preparation | Suspend 19.75 grams in 500 ml purified/distilled water. Adjust pH to 7.4 + 0.2 at 25 °C. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 40-50°C and aseptically add 5-7% v/v sterile lysed horse blood or 10% sterile defibrinated sheep blood and rehydrated contents of one vial of Blaser-Wang Selective Supplement, or Skirrow Selective Supplement. Mix well before pouring into sterile Petri plates. |
| Storage | BioScien Campylobacter Agar Base should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Campylobacter Agar Base is Cream to yellow homogeneous free flowing powder. Prepared medium: Basal medium: Yellow coloured clear gel. After addition of 5-7% v/v lysed blood: Reddish brown coloured opaque gel forms in Petri plates. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical samples - faeces; Food and dairy samples; Environmental samples. |
| Pack sizes | 3 options |
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