Microbiology

Cetrimide Agar

This medium is recommended as a selective medium for the isolation of Pseudomonas aeruginosa from pharmaceutical products during microbial limit testing.

Range	Microbiology
Composition	Formula per liter of medium g/l: peptone 20.0; Magnesium chloride 1.40; Dipotassium sulphate 10.0; Cetramide 0.3; Agar 13.6; Final pH 7.2 ± 0.2; ** Formula adjusted, standardized to suit performance parameters.
Preparation	Suspend 45.3 grams in 1000 ml purified/distilled water containing 10 ml glycerin/glycerol. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes or as per validated cycle. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Storage	BioScien Cetrimide Agar Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Cetrimide Agar is Cream to yellow homogeneous free flowing powder. Colour and Clarity of prepared medium is Light amber coloured opalescent gel with a slight precipitate forms in Petri plates.
Pack sizes	3 options