Bacteriology Media

Deoxycholate Agar

Deoxycholate Agar is a selective and differential medium employed for the enumeration of coliform bacteria in dairy products. It is also widely used for the isolation and differentiation of enteric pathogens from rectal swabs, faecal samples, and various clinical specimens.

Range	Bacteriology Media
Composition	Formula per liter of medium: Peptone 10.00 gm.; Lactose 10.00 gm.; Sodium deoxycholate 1.000 gm.; Sodium chloride 5.000 gm.; Dipotassium phosphate 2.000 gm; Ferric citrate 1.000 gm; Sodium citrate 1.000 gm; Neutral red 0.030 gm; Agar 15.000 gm. Final pH 7.3 ± 0.2 at 25°C
Preparation	Suspend 45.03 grams in 1000 ml distilled water. Adjust pH to 7.3 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Avoid excessive or prolonged heating during reconstitution. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Storage	BioScien Deoxycholate Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Deoxycholate agar is Light yellow to pink homogeneous free flowing powder. Prepared Media is Reddish orange coloured, clear to slightly opalescent gel. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
Specimen	Clinical: Stool and urine specimens. Food: Dairy products.
Pack sizes	3 options