Bacteriology Media

Desoxycholate Citrate Agar

Recommended for the selective isolation of enteric pathogens, particularly Salmonella and Shigella species, from clinical specimens.

Range	Bacteriology Media
Composition	Formula per liter of medium: Heart Infusion solids 10.00 gm.; Proteose Peptone 10.00 gm.; Lactose 10.00 gm.; Sodium Citrate 20.00 gm.; Ferric Ammonium Citrate 2.000 gm.; Sodium Desoxycholate 5.000 gm.; Agar 13.50 gm.; Neutral Red 0.020 gm. Final pH (at 25°C) 7.5±0.2
Preparation	Suspend 70.52 g of the powder in 1 L of purified water. Mix thoroughly. And adjust pH to 7.5 ± 0.2 at 25°C. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Pour into sterile perti plates. Avoid overheating. DO NOT AUTOCLAVE.
Storage	BioScien desoxycholate citrate agar dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien desoxycholate citrate is Light yellow to Pinkish-beige, free-flowing, homogeneous powder. If there are any physical changes, discard the medium. The hydrated medium is Orange-red, slightly opalescent gel forms in Petri plates, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations.
Specimen	Clinical- stool samples, urine samples; Food samples.
Pack sizes	3 options