Arena BioScien
Bacteriology Media
D.C.L.S. Agar
BS.1/DCLS01.100.0100
Fits your perfection
Bacteriology Media
D.C.L.S. Agar
D.C.L.S. Agar is used as a selective medium for the isolation of Shigella and Salmonella. Also useful for isolation of Vibrio cholera.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Proteose peptone 7.000 gm; Beef extract 3.000 gm; Lactose 5.000 gm; Sucrose 5.000 gm; Sodium citrate 10.00 gm; Sodium thiosulphate 5.000 gm; Sodium deoxycholate 2.500 gm; Neutral red 0.030 gm; Agar 12.00 gm. Final pH at 7.2 + 0.2 at 25 °C. # Equivalent to Meat extract B |
| Preparation | Suspend 49.53 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50°C and pour about 20 ml of medium into sterile Petri plates and allow to dry for about two hours with covers partially removed. |
| Storage | BioScien D.C.L.S. Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien D.C.L.S. Agar is Light yellow to pink homogeneous free flowing powder. Prepared Reddish orange coloured, clear to slightly opalescent gel forms in Petri plates. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical samples, stool samples. |
| Pack sizes | 3 options |
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