Bacteriology Media

Mueller Hinton Broth

Mueller Hinton Broth is recommended to determine the susceptibility of bacteria to sulphonamides by the tube dilution method. Also used for primary isolation of gonococci and meningococci.

Range	Bacteriology Media
Composition	Formula per liter of medium: Beef infusion 2; Casein hydrolysate 17.5; Starch 1.5; Final pH 7.3 ± 0.2 at 25°C
Preparation	Suspend 21 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Mix well and dispense into tubes as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Note: It is suggested to boil the medium before autoclaving to avoid settling of starch at the bottom.
Storage	BioScien Mueller Hinton Broth dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Mueller Hinton Broth is Cream to yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium is Light amber coloured clear solution in tubes, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations.
Specimen	Clinical samples
Pack sizes	3 options