Bacteriology Media
Mycoplasma Agar Base (PPLO Agar Base)
A selective medium for the isolation and cultivation of Mycoplasma Sp.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Beef heart, infusion from 6 gm; Peptone 10 gm; Sodium Chloride 5 gm; Agar 15 gm; Final pH 7.8±0.2 at 25°C |
| Preparation | 1- Suspend 36 gm of the dry powder in 700 ml distilled water. Mix thoroughly. Heat with frequent stirring and boil for 1 minute until the powder is completely dissolved. 2- Autoclave at 121°C for 15 minutes. Cool the medium to 50-60°C. 3- Aseptically add 300 ml 1% horse serum to the medium. Mix well. 4- Add Selective agents if desired (i.e. 0.25gm Thallium Acetate, 2.5mg Amphotericin B and 1,000,000 U Penicillin). Inoculate the surface of plates containing the complete medium by adding drops of liquid inoculum prepared by serial dilution of the sample, or by a swab-inoculation method. Incubate the media plates at 35 ± 2°C for up to 28 days under a moist atmosphere containing 5-10% carbon dioxide or anaerobically if the presence of M. buccale, M. faucium, M. orale or M. salivarium is suspected. |
| Storage | BioScien Mycoplasma Agar Base should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Mycoplasma Agar Base is cream to yellow homogeneous free flowing powder. Prepared Media is light yellow in color. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical samples- Urine sample, urethral swab, sputum sample; pharmaceutical samples |
| Pack sizes | 3 options |
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