Arena BioScien
Bacteriology Media
Mueller Kauffman Tetrathionate Broth Base
BS.1/MK01.100.0100
Fits your perfection
Bacteriology Media
Mueller Kauffman Tetrathionate Broth Base
Mueller Kauffman Tetrathionate Broth is used for improved enrichment and isolation of Salmonellae.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Casein enzymic hydrolysate 7 gm; Papaic digest of soyabean meal 2.3 gm; Sodium chloride 2.3 gm; Calcium carbonate 25 gm; Sodium thiosulphate 40.7 gm; Ox bile 4.75 gm; Final pH 8.0 ± 0.2 at 25°C |
| Preparation | Suspend 82.05 grams in 1000 ml distilled water. Adjust pH to 8.0 ± 0.2 at 25°C. Heat the medium just to boiling. DO NOT AUTOCLAVE. Cool and just before use aseptically add 19 ml of iodine solution (20 g iodine and 25 g potassium iodide in 100 ml sterile distilled water) and 9.5 ml of 0.1% brilliant green solution. Mix well to disperse calcium carbonate uniformly before dispensing in sterile tubes. Note: Due to presence of calcium carbonate, the prepared media forms opalescent solution with white precipitate. |
| Storage | BioScien Mueller Kauffman Tetrathionate Broth should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Mueller Kauffman Tetrathionate Broth is Cream to yellow homogeneous free flowing powder. Prepared Media With added brilliant green and iodine solution – Light green coloured opalescent solution forms with heavy white precipitate. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical samples (e.g., faeces, blood, and wounds). Food sample (milk and other dairy products) |
| Pack sizes | 3 options |
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