Bacteriology Media

Nutrient Broth No.2

For cultivation and enrichment of less fastidious bacteria and as a base in the preparation of special media.

RangeBacteriology Media
CompositionFormula per liter of medium: Meat peptone 4.300 gm; Tryptone 4.300 gm; Sodium Chloride 6.400 gm; Final pH 7.5 ± 0.2 at 25°C
PreparationSuspend 15 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
StorageBioScien Nutrient Broth No.2 should be stored between 10-30°C in a firmly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
AppearanceThe color of BioScien Nutrient Broth No.2 is Cream to yellow homogeneous free flowing powder. Prepared Media is Light yellow coloured clear solution. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard medium.
SpecimenClinical samples - faeces, urine; Food and dairy samples; Water samples.
Pack sizes3 options

Ordering information

Cat. No. (REF) Pack size Quote
BS.1/NN01.100.0100 100 Gram Quote
BS.1/NN01.250.0250 250 Gram Quote
BS.1/NN01.500.0500 500 Gram Quote
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