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Phenol Red Lactose Broth
Bacteriology Media

Phenol Red Lactose Broth

A broth medium that is recommended for lactose fermentation studies and detection of coliform bacteria.

RangeBacteriology Media
CompositionFormula per liter of medium: Peptone 10 gm; Beef Extract 1 gm; Sodium Chloride 5 gm; Lactose 5 gm; Phenol Red 0.018 gm; Final pH 7.4±0.2 at 25°C
PreparationSuspend 21 grams in 1000 ml distilled water, mix well. Heat if necessary to ensure complete solution. Distribute in tubes containing inverted Durham's tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
StorageBioScien Phenol Red Lactose Broth should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
AppearanceThe color of BioScien Phenol Red Lactose Broth is light yellow to pink colored homogeneous free flowing powder. Prepared Media is red in color without any precipitate. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
SpecimenWater samples, food samples and dairy products.
Pack sizes3 options
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Ordering information

Cat. No. (REF) Pack size
BS.1/PRL01.100.0100 100 Gram Quote
BS.1/PRL01.250.0250 250 Gram Quote
BS.1/PRL01.500.0500 500 Gram Quote
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