Arena BioScien
Bacteriology Media
Tryptose Blood Agar Base
BS.1/NZ01.100.0100
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Bacteriology Media
Tryptose Blood Agar Base
Tryptose Blood Agar Base is designed for the isolation of fastidious organisms and estimation the haemolytic reactions.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Tryptone 10.00 gm; HM Peptone B# 3.00 gm; Sodium Chloride 5.00 gm; Agar 15.00 gm. Final pH (at 25°C) 7.0 ± 0.2. # - Equivalent to beef extract. ** Formula adjusted, standardized to suit performance parameters. |
| Preparation | Suspend 33.0 grams in 950 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For preparing Blood Agar, cool the autoclaved medium to 45-50°C and aseptically add 5% v/v sterile defibrinated blood. Mix thoroughly, avoiding air bubbles and pour into sterile Petri plates. |
| Storage | BioScien Tryptose Blood Agar Base should be Stored between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Tryptose Blood Agar Base Cream to yellow homogeneous free flowing powder. prepared medium Basal medium : Yellow coloured clear to slightly opalescent gel forms in Petri plates. After addition of 5% v/v sterile defibrinated blood : Cherry red coloured opaque gel forms in Petri plates. |
| Pack sizes | 3 options |
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