Arena BioScien
Bacteriology Media
Urea Agar Base
BS.1/UA01.100.0100
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Bacteriology Media
Urea Agar Base
Recommended for the identification of bacteria on the basis of urea utilization, specifically for the differentiation of Proteus species from Salmonella and Shigella species.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptone 1.50 gm; Dextrose (Glucose) 1.00 gm; Sodium chloride 5.00 gm; Monopotassium phosphate 2.00 gm; Phenol red 0.012 gm; Agar 15.00 gm |
| Preparation | Urea Agar media is prepared by suspend 24.51 grams of the medium in 950 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 50 ml of sterile 40% Urea Solution and mix well. Dispense into sterile tubes and allow to set in the slanting position. Do not overheat or reheat the medium as urea decomposes very easily. |
| Storage | BioScien Urea Agar Base media are stable until expiration date stated on label when properly stored at 10-30°C. |
| Appearance | BioScien Urea Agar Base medium is Light pink coloured homogeneous free flowing powder. Prepared Media is Yellowish orange coloured clear gel forms in tubes as slants. If there are any physical changes, discard the medium. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Pure isolate from clinical, food and water samples. |
| Pack sizes | 3 options |
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