Bacteriology Media
C. L. E. D. with Andrade Indicator
C.L.E.D. Medium w/Andrade Indicator is recommended for isolation and differentiation of urinary pathogens on the basis of lactose fermentation.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptone 4.000 gm.; Beef extract 3.000 gm.; Tryptone 4.000 gm.; Lactose 10.00 gm.; L-Cystine 0.128 gm.; Bromothymol Blue 0.020 gm.; Andarde Indicator 0.100 gm.; Agar 15.00 gm.; Final pH 7.5 ± 0.2 at 25°C |
| Preparation | Suspend 36.25 grams in 1000 ml of distilled water. Adjust pH to 7.3 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates. |
| Storage | BioScien C. L. E. D. with Andrade Indicator dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien C. L. E. D. with Andrade Indicator is Light yellow to greyish yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium is Greenish blue clear to slightly opalescent gel forms in Petri plates, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Clinical: Urine sample |
| Pack sizes | 3 options |
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