Microbiology

Dextrose Peptone Broth

Dextrose Peptone Broth is designed for the cultivation of fastidious organisms, enumeration of thermophilic bacteria from canned foods and for routine sterility testing.

Range	Microbiology
Composition	Formula per liter of medium: Peptone 20.00 gm; Dextrose 10.00 gm; Sodium chloride 5.000 gm. Final pH 7.2 ± 0.2 at 25°C
Preparation	Suspend 35 grams in 1000 ml distilled water. Adjust pH to 7.2 ± 0.2 at 25°C. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes.
Storage	BioScien Dextrose peptone broth should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Dextrose peptone broth is Cream to light yellow homogeneous free flowing powder. Prepared Media is Light yellow coloured, clear to slightly opalescent solution in tubes. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
Specimen	Food samples; Water and sewage samples.
Pack sizes	3 options