Bacteriology Media
Edwards Medium Base
Recommended for selective and rapid isolation of Streptococcus agalactiae and other Streptococci associated with bovine mastitis.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptone 10.00 gm; Beef extract 10.00 gm; Esculin 1.00 gm; Sodium chloride 5.00 gm; Crystal violet 0.0013 gm; Thallous sulphate 0.330 gm; Agar 15.00 gm; Final pH 7.4 ± 0.2 at 25°C |
| Preparation | 41.33 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 115°C for 20 minutes. Cool to 45-50°C and aseptically add 5 to 7% v/v sterile sheep blood. Mix well and pour into sterile Petri plates. |
| Storage | BioScien Edwards Medium Base are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Edwards Medium Base is Cream to yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. Basal medium: Amber coloured, clear to slightly opalescent gel. After addition of 5-7% v/v sterile defibrinated sheep blood: Cherry red coloured opaque gel forms in Petri plates, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Clinical samples - faeces, vaginal swabs; Dairy samples. |
| Pack sizes | 3 options |
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