Bacteriology Media
Egg Yolk Agar Base
Recommended for isolation and identification of Clostridia and certain other anaerobes.
| Range | Bacteriology Media |
|---|---|
| Composition | Per Liter: Pancreatic Digest of Casein 10.0 gm; Beef Extract 3.0 gm; Yeast Extract 1.0 gm; Sodium Chloride 5.0 gm; Agar 15.0 gm; Final pH 7.4 ± 0.2 at 25°C |
| Preparation | Hydrated Egg yolk Agar media is prepared by Suspend 34 grams in 900 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Dispense 90 ml amounts in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45-50°C and add 10 ml of sterile egg yolk emulsion per 90 ml of medium. Mix well and pour into sterile Petri plates. Adjust the pH to 7.4+0.2, Pour into a sterile petri dishes. Prior to inoculation, media plates should be reduced by placing in an anaerobic jar for 18-24 hours. An enrichment broth should be simultaneously inoculated with the test sample to detect small number of anaerobic organisms. Standard procedures for the isolation of organism should be referred. Incubation should be carried out for 18-48 hours and continued for 7 days. After use, contaminated materials must be sterilized by autoclaving before discarding. |
| Storage | BioScien Egg yolk Agar dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. |
| Appearance | The color of BioScien Egg yolk Agar medium is cream to yellow homogeneous free flowing powder, dehydrated medium is Basal medium: Medium amber coloured, clear to slightly opalescent gel After addition of egg yolk emulsion: Yellow coloured opaque gel forms in Petri plates If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical- stool, abscess, etc.; Food samples |
| Pack sizes | 3 options |
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