Bacteriology Media
EMB Agar
For the selective and differentiation of Gram-negative enteric bacteria from clinical and non-clinical specimens.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptone 10.0; Dipotassium hydrogen phosphateDextrose 2.0; Lactose 5.0; Sucrose 5.0; Eosin-Y 0.400; Methylene blue 0.065; Agar 13.5. Final pH 7.2 ± 0.2 at 25°C |
| Preparation | Suspend 35.96 grams in 1000 ml purified / distilled water. Mix until suspension is uniform. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING. Cool to 45-50°C and shake the medium in order to oxidize the methylene blue (i.e. to restore its blue colour) and to suspend the flocculent precipitate. (If EMB Agar is inoculated on the same day, it may be used without autoclave sterilization). Precaution: Store the medium away from light to avoid photooxidation. |
| Storage | BioScien Eosin Methylene Blue (EMB) Agar dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien EMB Agar is Light pink to purple homogeneous free flowing powder. If there are any physical changes, discard the medium. Colour and Clarity of prepared medium Reddish purple coloured, opalescent gel with greenish cast and finely dispersed precipitate forms in Petri plates. |
| Specimen | Food samples; Water and sewage samples |
| Pack sizes | 3 options |
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