Bacteriology Media
Endo Agar
For confirmation of the presumptive test for members of the coliform group from clinical and non-clinical samples.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptone 10.00 gm; Lactose 10.00 gm; Dipotassium hydrogen phosphate 3.500 gm; Sodium sulphite 2.500 gm; Basic fuchsin 0.500 gm; Agar 15.00 gm; Final pH 7.5 ± 0.2 at 25°C |
| Preparation | Suspend 41.5 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before pouring into sterile Petri plates. If the solidified culture medium is somewhat too red, then to remove the colour add a few drops (max. 1 ml/litre) of a freshly prepared 10% Sodium sulphite solution and boil. |
| Storage | BioScien Endo Agar dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Endo Agar medium is Light pink to purple homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium is Orangish pink coloured, clear to slightly opalescent gel with fine precipitate forms in Petri plates, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Clinical samples - urine; Food and dairy samples; Water samples. |
| Pack sizes | 3 options |
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