Bacteriology Media
Mannitol Salt Broth
Mannitol Salt Broth is used for the selective isolation of presumptive pathogenic Staphylococci.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula in g/L: Peptic digest of animal tissue 10; Beef extract 1; Sodium chloride 75; Mannitol 10; Phenol red 0.025; pH 6.8 ± 0.2 at 25°C |
| Preparation | Suspend 96.02 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Note: This product contains 7.5% sodium chloride as one of its ingredients. On repeated exposure to air and absorption moisture sodium chloride has tendency to form lumps, therefore we strongly recommend storage in tightly closed containers in dry place away from bright light. |
| Storage | BioScien Mannitol Salt Broth should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within the stated expiry period. |
| Appearance | BioScien Mannitol salt broth is Light yellow to pink homogeneous free flowing powder. Prepared medium is Red coloured clear solution in tubes. should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | For clinical samples follow appropriate techniques for handling specimens as per established guidelines (11,12). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (9,10,13). After use, contaminated materials must be sterilized by autoclaving before discarding. Clinical samples; Food and Dairy samples; Environmental samples |
| Pack sizes | 3 options |
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