Bacteriology Media
Milk Agar with Cetrimide
Milk Agar with Cetrimide is used for the detection and enumeration of Pseudomonas aeruginosa in swimming pool waters.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Part A - Skim milk powder 133.33 gm.; Part B - Peptic digest of animal tissue 3.330 gm.; Sodium chloride 1.670 gm.; Yeast extract 1.000 gm.; Cetrimide 0.400 gm.; Agar 20.000 gm.; Final pH 7.3 ± 0.2 at 25°C |
| Preparation | Suspend 26.4 grams of Part B in 250 ml distilled water. Adjust pH to 7.3 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 20 minutes. Suspend 133.33 grams of Part A in 750 ml of distilled water. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 5 minutes. After autoclaving mix Part A and B and pour into sterile Petri plates. |
| Storage | BioScien Milk Agar with Cetrimide dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien milk agar with cetrimide appearance of Part A: White to cream homogeneous free flowing powder. Part B: Cream to yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium is Light amber coloured opalescent gel forms in Petri plates, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Clinical samples. |
| Pack sizes | 3 options |
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