Bacteriology Media

MIO Medium (Motility Indole Ornithine Medium)

Designed for the identification of Enterobacteriaceae on the basis of motility, ornithine decarboxylase activity and indole production.

Range	Bacteriology Media
Composition	Formula per liter of medium: Tryptone 10.00 gm; peptone 10.00 gm; Yeast extract 3.00 gm; Dextrose 1.00 gm; L-Ornithine hydrochloride 5.00 gm; Bromocresol purple 0.02 gm; Agar 2.00 gm. Final pH 6.5 ± 0.2. ** Formula adjusted, standardized to suit performance parameters.
Preparation	Suspend 31.02 g of the medium in 1 L of purified/distilled water. Heat to boiling until completely dissolved. Dispense 5 mL into test tubes and autoclave at 121°C (15 psi) for 15 minutes. Cool the tubes in an upright position before use.
Storage	BioScien MIO Medium Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien MIO Medium Is Light yellow to pale green homogeneous free flowing powder. Prepared medium is purple coloured clear to slightly opalescent gel forms in tubes as butts.
Pack sizes	3 options