Bacteriology Media

MRS (deMan, Rogosa, Sharpe) Agar

For the enrichment, cultivation and isolation of all species of Lactobacillus from all types of material according to DeMan, Rogosa and Sharpe.

Range	Bacteriology Media
Composition	Formula per liter of medium: Universal peptone 10; Meat extract 5; Yeast extract 5; D(+)-Glucose 20; Dipotassium hydrogen phosphate 2; Diammonium hydrogen citrate 2; Sodium acetate 5; Magnesium sulfate 0.1; Manganous sulfate 0.05; Agar 12; Final pH 6.5 +/- 0.2 at 25°C
Preparation	Dissolve 61.15 g in distilled water, add 1 ml Tween 80 and bring volume to 1000 ml. Boil to dissolve the medium completely. Autoclave at 121°C for 15 minutes. Incubate the culture up to 3 days at 35°C or up to 5 days at 30°C. If possible, incubate the plates in a CO2-enriched atmosphere in an anaerobic jar. Do not allow the surface of the plates to dry as this will cause acetate concentration increasing at the surface, which inhibits the growth of lactobacilli.
Storage	BioScien Store between 10-30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien MRS Agar is Cream to light yellow homogeneous free flowing powder. Prepared Media is Medium to dark amber coloured, clear to slightly opalescent gel forms in Petri plates. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
Specimen	Clinical samples - urine, faeces, etc.; Food and dairy samples
Pack sizes	3 options