Bacteriology Media

Potato Dextrose Broth

Potato Dextrose Broth is recommended for the isolation and enumeration of yeasts and moulds.

RangeBacteriology Media
CompositionFormula per liter of medium: Potato infusion 4.00 gm; Dextrose 20.0 gm; Final pH 5.2 ± 0.2 at 25°C
PreparationSuspend 24 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before dispensing. In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml. of sterile, cooled medium is approximately 1ml. Do not heat the medium after addition of the acid.
StorageBioScien Potato Dextrose Broth should be stored between 10-30°C in a firmly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
AppearanceThe color of BioScien Potato Dextrose Broth is Off-white to yellow homogeneous free flowing powder. Prepared Media is Light amber coloured clear to slightly opalescent solution in tubes. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
SpecimenFood samples; Water and sewage samples
Pack sizes3 options

Ordering information

Cat. No. (REF) Pack size Quote
BS.1/DB01.100.0100 100 Gram Quote
BS.1/DB01.250.0250 250 Gram Quote
BS.1/DB01.500.0500 500 Gram Quote
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