Microbiology

Purple Agar Base

Purple Agar Base is recommended for the preparation of carbohydrate media used in fermentation studies for the cultural identification of pure cultures of enteric and other microorganisms.

RangeMicrobiology
CompositionFormula per liter of medium: Peptone special 10 gm.; Beef extract 1 gm.; Sodium Chloride gm.; Bromocresol purple gm.; Agar 15 gm.; Final pH 6.8 ± 0.2 at 25°C
PreparationSuspend 31.02 grams in 1000 ml distilled water. Add 5 - 10 grams of the carbohydrate to be tested. Heat to boiling to dissolve the medium completely. Dispense in tubes as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Alternatively sterilize the basal medium prepared using 900 ml distilled water and add 100 ml separately sterilized 5 - 10% solution of the desired carbohydrate to it.
StorageBioScien Purple agar base should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
AppearanceThe color of BioScien Purple agar base is Cream to greenish yellow homogeneous free flowing powder. Prepared Medium Purple coloured clear to slightly opalescent gel forms in tubes as slants clear solution in tubes. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
SpecimenClinical sampels
Pack sizes3 options

Ordering information

Cat. No. (REF) Pack size Quote
BS.1/PU01.100.0100 100 Gram Quote
BS.1/PU01.250.0250 250 Gram Quote
BS.1/PU01.500.0500 500 Gram Quote
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