Bacteriology Media
Reinforced Clostridial Medium
Reinforced Clostridial Medium is used for cultivating and enumerating clostridia, other anaerobes, and other species of bacteria from foods and clinical specimens.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Bacteriological agar 0.5 gm; Glucose monohydrate 5 gm; Peptone 10 gm; Cysteine hydrochloride 0.5 gm; Beef extract 10 gm; Yeast extract 3 gm; Sodium acetate 3 gm; Soluble starch 1 gm; Sodium chloride 5 gm; Final pH 6.8 ± 0.2 at 25°C |
| Preparation | Suspend 38.00 grams in 1000 ml distilled water. Adjust pH to 6.8 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Pour into appropriate containers. |
| Storage | BioScien Reinforced Clostridial medium should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Reinforced Clostridial medium is Cream to yellow homogeneous free flowing powder. Prepared Media is Upon cooling, amber and becomes more opalescent. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical samples (e.g., faeces, blood, and wounds); Pharmaceutical samples; Food sample (milk and other dairyproducts) |
| Pack sizes | 3 options |
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