Bacteriology Media

S.S Agar

Medical laboratories media recommended for differential and selective isolation of Salmonella and Shigella species from pathological specimens, suspected foodstuffs etc.

Range	Bacteriology Media
Composition	Concentration g/l: Peptone 5.000; HM peptone B # 5.000; Lactose 10.000; Bile salts mixture 8.500; Sodium citrate 10.000; Sodium thiosulphate 8.500; Ferric citrate 1.000; Brilliant green 0.00033; Neutral red 0.025; Agar 15.000; Final pH ( at 25°C) 7.0±0.2
Preparation	SS Agar media is prepared by suspend 63 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Don't autoclave or overheat. Cool to about 50°C, mix and pour into sterile Petri dishes. Final pH 7.0 ± 0.2 at 25°C
Storage	BioScien SS Agar media are stable until expiration date stated on label when properly stored 10-30°C.
Appearance	The color of BioScien SS Agar medium is cream to yellow homogeneous free flowing powder, dehydrated medium is clear light amber coloured to slightly opalescent gel. After addition of 5% v/v sterile defibrinated blood it turn to cherry red coloured, opaque gel forms in Petri plates. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium..
Specimen	For clinical samples follow appropriate techniques for handling specimens as per established guidelines. For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines. After use, contaminated materials must be sterilized by autoclaving before discarding.
Pack sizes	3 options