Bacteriology Media

Selenite Broth Base

Selenite Broth Base w/o selenite is recommended for the enrichment of Salmonellae species from food, dairy products and pathological specimens, on addition of selenite.

Range	Bacteriology Media
Composition	Formula per liter of medium: Part A: Skim milk powder 133.33 gm.; Part B: Peptic digest of animal tissue 3.330 gm.; Sodium chloride 1.670 gm.; Yeast extract 1.000 gm.; Cetrimide 0.400 gm.; Agar 20.000 gm.; Final pH 7.0 ± 0.2 at 25°C
Preparation	Suspend 4 grams of sodium hydrogen selenite in 1000 ml distilled water. Adjust pH to 7.0 ± 0.2 at 25°C. Add 19 grams of selenite broth base. Mix well. Warm to dissolve the medium completely. Distribute in sterile test tubes. Sterilize in a boiling water bath or free flowing steam for 10 minutes. DO NOT AUTOCLAVE. Excessive heating is detrimental. Discard the prepared medium if large amount of selenite is reduced (indicated by red precipitate at the bottom of tube/bottle).
Storage	BioScien Selenite broth base dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Selenite broth base is Cream to light yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium is Cream to yellow coloured clear solution without any precipitate, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations.
Specimen	Clinical samples - Blood; Food and dairy samples
Pack sizes	3 options