Bacteriology Media

Sabouraud Dextrose Agar (SDA)

Medical laboratories medium used for the cultivation of yeasts, moulds and aciduric bacteria, particularly useful for the fungi associated with skin infections and other clinical specimens.

Range	Bacteriology Media
Composition	Formula per liter of medium: Mycological peptone 10 gm; Dextrose 40 gm; Agar 15 gm; Final pH 5.6 ± 0.2 at 25°C
Preparation	Suspend 65 grams in 1 liter of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 minutes.
Storage	BioScien Sabouraud Dextrose Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Sabouraud Dextrose Agar is light yellow homogeneous free flowing powder. Prepared Media is light amber in color. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
Specimen	All Clinical specimens that require Fungi detection.
Pack sizes	3 options