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Selenite F Broth
Bacteriology Media

Selenite F Broth

Selective enrichment medium for the isolation of Salmonellae and some Shigella from faeces, urine, and other pathological specimens.

RangeBacteriology Media
CompositionFormula per liter of medium: Tryptone 5 gm; Lactose 4 gm; Dipotassium hydrogen phosphate 3.5 gm; Potassium dihydrogen phosphate 6.5 gm; Sodium selenite 4 gm; Final pH 7.0 ± 0.2 at 25°C
PreparationDissolve 23 grams in 1 liter of distilled water. Warm to dissolve the medium completely. Distribute in sterile test tubes. Sterilize in a boiling water bath or free flowing steam for 10 minutes. Discard the prepared medium if large quantities of selenite are reduced (shown by red precipitate at the bottom of tube/bottle). DO NOT AUTOCLAVE.
StorageBioScien Selenite F Broth should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
AppearanceThe color of BioScien Selenite F Broth is white to yellow homogeneous free flowing powder. Prepared Media is cream to yellow in color. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
Specimen-Clinical samples (e.g. Feces, urine or other pathological specimens).
Pack sizes3 options
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Ordering information

Cat. No. (REF) Pack size
BS.1/SF01.100.0100 100 Gram Quote
BS.1/SF01.250.0250 250 Gram Quote
BS.1/SF01.500.0500 500 Gram Quote
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