Bacteriology Media

Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS Agar)

Recommended for the selective isolation and cultivation of Vibrio Sp. and differentiating between them

Range	Bacteriology Media
Composition	Formula per liter of medium: Yeast extract 5 gm; Peptone 10 gm; Sodium thiosulphate 10 gm; Sodium citrate 10 gm; Bile salts 8 gm; Sucrose 20 gm; Sodium chloride 10 gm; Ferric citrate 1 gm; Bromothymol blue 0.04 gm; Thymol blue 0.04; Agar 15 gm; Final pH 8.6 ± 0.2 at 25°C
Preparation	Suspend 89.08 grams in 1 liter distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Storage	BioScien TCBS Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien TCBS Agar is light yellow homogeneous free flowing powder. Prepared Media is bluish green in color. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
Specimen	Faecal specimens, food specimens and water specimens.
Pack sizes	3 options