Bacteriology Media
Tryptic Soy Agar
A general-purpose media that supports the growth of gram-positive and gram-negative
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Soya Peptone 3 gm; Tryptone 17 gm; Sodium Chloride 5 gm; Glucose 2.5 gm; Dipotassium Hydrogen phosphate 2.5 gm; Agar 15 gm; Final pH 7.3±0.2 at 25°C |
| Preparation | Suspend 45 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates. |
| Storage | BioScien Tryptic Soya Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Tryptic Soya Agar is cream to yellow homogeneous free flowing powder. Prepared Media is light yellow in color. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Food and dairy samples; Water samples |
| Pack sizes | 3 options |
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