Bacteriology Media
Tryptone Soya Yeast Extract Agar (TSYEA)
Tryptone Soya Yeast Extract Broth is recommended for confirmation of Listeria in Henrys light.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Casein enzymic hydrolysate 17.00 gm.; Papaic digest of soyabean meal 3.000 gm.; Sodium chloride 5.000 gm.; Dipotassium hydrogen phosphate 2.500 gm.; Dextrose 2.500 gm.; Yeast extract 6.000 gm.; Agar 15.00 gm.; Final pH 7.3 ± 0.2 at 25°C |
| Preparation | Suspend 51 grams in 1000 ml distilled water. Adjust pH to 7.3 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates. |
| Storage | BioScien Tryptone Soya Yeast Extract Agar dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Tryptone Soya Yeast Extract Agar is Cream to yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium is Yellow coloured clear to slightly opalescent gel forms in Petri plates, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Clinical samples |
| Pack sizes | 3 options |
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