Microbiology

Triple Sugar Iron Agar

Recommended for the identification of gram-negative enteric bacilli on the basis of dextrose, lactose and sucrose fermentation and hydrogen sulphide production.

Range	Microbiology
Composition	Formula per liter of medium: Beef extract 3.0 gm; Peptone 20.0 gm; Yeast extract 3.0 gm; Lactose 10.0 gm; Sucrose 10.0 gm; Dextrose 1.0 gm; Sodium chloride 5.0 gm; Sodium thiosulphate 0.3 gm; Ferrous citrate 0.3 gm; Phenol red 0.024 gm; Agar 12.0 gm; Final pH after sterilization 7.4 ± 0.2 at 25°C
Preparation	Suspend 64.52 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Mix well and distribute into test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the medium to set in sloped form with a butt about 1 inch long. Note: For better results, the medium can be sterilized by autoclaving at 10 lbs pressure (115°C) for 15 minutes.
Storage	BioScien Triple Sugar Iron Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Triple Sugar Iron Agar is Light yellow to pink homogeneous free flowing powder prepared medium is Pinkish red coloured clear to slightly opalescent gel forms in tubes as slants. If there are any physical changes or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for media, discard the medium.
Specimen	Isolated microorganism from water, food, or clinical sample.
Pack sizes	3 options