Microbiology

Todd Hewitt Broth

Todd Hewitt Broth is recommended for the cultivation of group A haemolytic streptococci used for serological studies.

RangeMicrobiology
CompositionFormula per liter of medium: Beef heart, infusion from 500.00 gm.; Peptic digest of animal tissue 20.00 gm.; Dextrose 2.000 gm.; Sodium chloride 2.000 gm.; Disodium phosphate 0.400 gm.; Sodium carbonate 2.500 gm.; Final pH 7.8 ± 0.2 at 25°C
PreparationSuspend 37 grams in 1000 ml distilled water. Adjust pH to 7.8 ± 0.2 at 25°C. Mix well and dispense as desired. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes.
StorageBioScien Todd Hewitt Broth dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
AppearanceThe color of BioScien Todd Hewitt Broth is Cream to yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium is Medium amber coloured clear solution without ant precipitate. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations.
SpecimenClinical samples: Blood
Pack sizes3 options

Ordering information

Cat. No. (REF) Pack size Quote
BS.1/HT01.100.0100 100 Gram Quote
BS.1/HT01.250.0250 250 Gram Quote
BS.1/HT01.500.0500 500 Gram Quote
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