Bacteriology Media

Thioglycollate Medium w/o Dextrose

Thioglycollate Medium w/o Dextrose is employed for cultivating aerobes, microaerophiles, and anaerobes, as well as for conducting carbohydrate fermentation studies using various sugar sources.

Range	Bacteriology Media
Composition	Formula per liter of medium: Casein enzymic hydrolysate 15.00 gm; Sodium chloride 2.50 gm; Sodium thioglycollate 0.50 gm; L-Cystine 0.250 gm; Yeast extract 5.00 gm; Agar 0.750 gm. Final pH (at 25°C) 7.2 ± 0.2. ** Formula adjusted, standardized to suit performance parameters.
Preparation	Suspend 24.00 grams in 1000 ml distilled water. If the medium is to be used for fermentation studies or for diagnostic work add 0.5 to 1% carbohydrate of choice. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Alternatively, sterile carbohydrate solutions may be added to the broth after sterilization. The prepared medium should be stored in the dark at room temperature.
Storage	BioScien Thioglycollate Medium without Dextrose Store below 30°C and the prepared medium at 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Thioglycollate Medium without Dextrose Cream to yellow homogeneous free flowing powder. prepared medium Light amber coloured, very slightly opalescent viscous solution with upper 10% or less medium green on standing.
Pack sizes	3 options