Bacteriology Media

Tryptone Yeast Extract Cystine w/Sucrose and w/o Bacitracin Agar (TYCSB)

Recommended for selective isolation of Streptococcus mutans.

RangeBacteriology Media
CompositionFormula per liter of medium: Tryptone 15.0 gm; Yeast extract 5.00 gm; Disodium hydrogen phosphate.7H2O 1.00 gm; Sodium bicarbonate 2.00 gm; Sodium acetate.3H2O 20.0 gm; Sucrose 200.0 gm; L-Cystine 0.200 gm; Sodium chloride 0.100 gm; Sodium sulphite 0.100 gm; Agar 15.00 gm; Final pH 7.3 ± 0.2 at 25°C
PreparationSuspend 249.99 grams (equivalent weight of dehydrated medium per litre) in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add sterile rehydrated contents of 1 vial of TYCSB supplement. Mix well and pour into sterile petri plates.
StorageBioScien TYCSB Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
AppearanceThe color of BioScien TYCSB Agar is Cream to yellow homogeneous free flowing powder. Prepared Media is Light yellow coloured clear to slightly opalescent gel forms in Petri plates. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
SpecimenClinical samples - Saliva Swab, Decaying tooth (4), etc.
Pack sizes3 options

Ordering information

Cat. No. (REF) Pack size Quote
BS.1/TY01.100.0100 100 Gram Quote
BS.1/TY01.250.0250 250 Gram Quote
BS.1/TY01.500.0500 500 Gram Quote
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