Microbiology
Tryptone Soya Yeast Extract Broth (TSYEB)
Tryptone Soya Yeast Extract Broth is recommended for confirmation of Listeria in Henrys light.
| Range | Microbiology |
|---|---|
| Composition | Formula per liter of medium: Casein enzymic hydrolysate 17.00 gm.; Papaic digest of soyabean meal 3.000 gm.; Sodium chloride 5.000 gm.; Dipotassium hydrogen phosphate 2.500 gm.; Dextrose 2.500 gm.; Yeast extract 6.000 gm.; Final pH 7.3 ± 0.2 at 25°C |
| Preparation | Suspend 41.5 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before pouring into sterile Petri plates. If the solidified culture medium is somewhat too red, then to remove the colour add a few drops (max. 1 ml/litre) of a freshly prepared 10% Sodium sulphite solution and boil. |
| Storage | BioScien Tryptone Soya Yeast Extract Broth dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Tryptone Soya Yeast Extract Broth is Cream to yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium is Yellow coloured clear solution in tubes, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Clinical samples |
| Pack sizes | 3 options |
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