Arena BioScien
Bacteriology Media
Ziehl-Neelsen Stain
BS.1/ZS03.100.0300
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Bacteriology Media
Ziehl-Neelsen Stain
Used in the differentiation of acid-fast bacteria.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Solution 1 - Carbol fuchsin stain; Solution 2 - Decolorizing solvent; Solution 3 - Counterstain |
| Procedure | 1. Heat fix an air dried smear at 80o C for at least 15 minutes or for 2 hours on an electric hot plate at 65o C – 70o C. 2. Place a slide with an air-dried and heat-fixed smear on suitable staining device. Cut a piece of absorbent paper to fit the slide and saturate the paper with the carbolfuchsin stain. 3. Carefully heat the underside of the slide by passing a flame under the rack or by placing the slide on a hot plate until steam rises (without boiling!). Keep the preparation moist with stain and steaming for 5 minutes, repeating the heating as needed. (CAUTION: overheating causes spattering of the stain and may crack the slide). 4. Wash the film in a gentle and indirect stream of tap water* until no color appears in the effluent. 5. Holding the slide with forceps, wash the slide with the decolorizing solvent. Immediately wash with tap water*, as above. Repeat the decolorizing and the washing until the stained smear appears faintly pink and the fluid washing off the slide runs clear. 6. Flood the smear with the methylene blue counterstain for 20 to 30 seconds, and wash with tap water*, as above. 7. Gently blot, or air dry the smear. 8. Examine under oil immersion. ** Acid-fast bacteria appear red, and non-acid-fast bacteria (and other organisms and cellular materials) appear blue. |
| Storage | BioScien Ziehl - Neelsen Stain are stable until expiration date stated on label when properly stored 10-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle. |
| Specimen | Clinical samples: Blood |
| Pack sizes | 1 option |
Ordering information
| Cat. No. (REF) | Pack size | Quote |
|---|---|---|
BS.1/ZS03.100.0300 |
3 X 100 ml | Quote |
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