Bacteriology Media

Amies Transport Medium without Charcoal

Transport Medium, Amies without Charcoal is used for transport and preservation of microbiological specimen.

RangeBacteriology Media
CompositionFormula per liter of medium: Sodium chloride 3.000 gm.; Potassium chloride 0.200 gm.; Calcium chloride 0.100 gm.; Magnesium chloride 0.100 gm.; Monopotassium phosphate 0.200 gm.; Disodium phosphate 1.150 gm.; Sodium thioglycollate 1.000 gm.; Agar 4.000 gm.; Final pH 7.3 ± 0.2 at 25°C
PreparationSuspend 9.75 grams in 1000 ml distilled water. Adjust pH to 7.3 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. Dispense in screw cap bottles or tubes in 6 ml or desired quantity. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool in an upright position. Turn the tubes several times while agar is solidifying, to maintain uniform suspension of charcoal particles.
StorageBioScien Amies transport medium without charcoal dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
AppearanceThe color of BioScien Amies transport medium without charcoal is Off-white to yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium Colorless clear to slightly opalescent gel forms in tubes as butts, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations.
SpecimenClinical samples
Pack sizes1 option

Ordering information

Cat. No. (REF) Pack size Quote
BS.1/AI01.500.0500 500 Gram Quote
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