Bacteriology Media

Azide Blood Agar Base

Is used for the selective isolation and cultivation of Streptococcus and Staphylococcus species from mixed bacterial flora in clinical and non-clinical samples.

Range	Bacteriology Media
Composition	Formula per liter of medium: Peptone, special 10.00 gm.; Beef extract 3.000 gm.; Sodium chloride 5.000 gm.; Sodium azide 0.200 gm.; Agar 15.00 gm. Final pH 7.2 ± 0.2 at 25°C
Preparation	Suspend 33.2 grams in 1000 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45-50°C. For preparing Blood Agar plates, 5% v/v sterile defibrinated blood is added aseptically. Mix well and pour into sterile Petri plates.
Storage	BioScien Azide Blood Agar base should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Azide Blood Agar Base is Cream to yellow homogeneous free flowing powder. Prepared Basal Medium is Yellow coloured, clear to slightly opalescent gel. After addition of 5%w/v sterile defibrinated blood is Cherry red coloured, opaque gel forms in Petri plates, which darkens on standing. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
Specimen	Clinical samples – Blood
Pack sizes	3 options