Bacteriology Media
Actidione Agar w/ Actidione
Actidione Agar w/ Actidione designed for the enumeration of bacteria in specimens containing large numbers of yeasts and moulds.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Tryptone 5.00 gm; Dextrose (Glucose) 50.00 gm; Yeast extract 4.00 gm; Potassium dihydrogen phosphate 0.550 gm; Potassium chloride 0.425 gm; Calcium chloride anhydrous 0.125 gm; Magnesium sulphate 0.125 gm; Ferric chloride 0.0025 gm; Manganese sulphate 0.0025 gm; Bromo cresol green 0.022 gm; Actidione (Cycloheximide) 0.010 gm; Agar 15.00 gm. Final pH (at 25°C) 5.5 ± 0.2. ** Formula adjusted, standardized to suit performance parameters. |
| Preparation | Suspend 75.26 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well before pouring into sterile Petri plates. |
| Storage | BioScien Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Actidione Agar is light yellow to light green, appearing as a homogeneous, free-flowing powder. Upon preparation, a greenish-blue, clear to slightly opalescent gel is formed in Petri plates. |
| Pack sizes | 3 options |
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