Bacteriology Media

Azide Dextrose Broth

For detection and enumeration of Streptococci in water, sewage, food and other materials suspected of sewage contamination.

RangeBacteriology Media
CompositionFormula per liter of medium: Peptone, special 15.00 gm; Beef extract 4.50 gm; Dextrose (Glucose) 7.50 gm; Sodium chloride 7.50 gm; Sodium azide 0.200 gm; Final pH 7.2 ± 0.2 at 25°C
PreparationSuspend 34.7 grams in 1000 ml purified/ distilled water for preparing single strength broth or use 69.4 grams in 1000 ml purified / distilled water for double strength broth. Heat, if necessary, to ensure complete solution. Dispense in test tubes and sterilize by autoclaving at 118°C for 15 minutes.
StorageBioScien Azide Dextrose Broth should be stored between 10-30°C in a firmly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
AppearanceThe color of BioScien Azide Dextrose Broth is Cream to yellow homogeneous free flowing powder. Prepared Media is Amber coloured clear solution without any precipitate. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
SpecimenFood samples; Water and sewage samples
Pack sizes3 options

Ordering information

Cat. No. (REF) Pack size Quote
BS.1/DB01.100.0100 100 Gram Quote
BS.1/DB01.250.0250 250 Gram Quote
BS.1/DB01.500.0500 500 Gram Quote
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